human premixed 29-plex magnetic luminex assay Search Results


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Merck KGaA milliplex map human cytokine/chemokine magnetic bead panel 29-plex
Milliplex Map Human Cytokine/Chemokine Magnetic Bead Panel 29 Plex, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation human il-12/il-23 p40 mab (clone 169516)
Human Il 12/Il 23 P40 Mab (Clone 169516), supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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DiaSorin Biotechnology 200 miliplex analyzer system
200 Miliplex Analyzer System, supplied by DiaSorin Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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10X Genomics feature barcode kit 10x genomics pn
Figure 1. Defining the immune landscape of term decidua (A) Experimental design: CD45+ leukocytes were isolated from maternal blood and decidua from term cesarean deliveries (n = 4). Single-cell suspensions of decidual leukocytes and matched PBMCs were subjected to gene expression profiling using <t>10x</t> 30 single-cell gene expression protocol (scRNA-seq).
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Figure 1. Defining the immune landscape of term decidua (A) Experimental design: CD45+ leukocytes were isolated from maternal blood and decidua from term cesarean deliveries (n = 4). Single-cell suspensions of decidual leukocytes and matched PBMCs were subjected to gene expression profiling using 10x 30 single-cell gene expression protocol (scRNA-seq).

Journal: Cell reports

Article Title: Multimodal profiling of term human decidua demonstrates immune adaptations with pregravid obesity.

doi: 10.1016/j.celrep.2023.112769

Figure Lengend Snippet: Figure 1. Defining the immune landscape of term decidua (A) Experimental design: CD45+ leukocytes were isolated from maternal blood and decidua from term cesarean deliveries (n = 4). Single-cell suspensions of decidual leukocytes and matched PBMCs were subjected to gene expression profiling using 10x 30 single-cell gene expression protocol (scRNA-seq).

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER MitoTracker Red CMXRos ThermoFisher Scientific Cat#M7512 Seahorse XF Cell Energy Phenotype kit Agilent Technologies Cat#103592-100 Human Premixed 29-plex Magnetic Luminex Assay R&D Systems Cat#Custom-LxSA-H-29 Lot#C0003514 TotalSeq B Hashtag Oligos 1-10 BioLegend 384631–392649 Chromium Single Cell 3ʹ Reagent Kits v3 10X Genomics PN-1000075 30 Feature Barcode Kit 10X Genomics PN-1000262 Chromium Single Cell 5ʹ Reagent Kits V2 10X Genomics PN-1000263 50 Feature Barcode Kit 10X Genomics PN-1000256 Deposited Data 30 Single Cell GEX data - Decidua This paper NCBI Sequence Read Archive: PRJNA817521 50 Single Cell TCR data - Decidua This paper NCBI Sequence Read Archive: PRJNA817521 50 Single Cell TCR data – Maternal PBMC This paper NCBI Sequence Read Archive: PRJNA817521 30 Single Cell GEX data -Villous This paper NCBI Sequence Read Archive:PRJNA946160 30 Single Cell GEX data - UCBMC Sureshchandra et al. 202166 10.3389/fimmu.2021.617592 NCBI Sequence Read Archive:PRJNA690128 30 Single Cell GEX data – Maternal PBMC This paper and Sureshchandra et al. 202166 10.1016/j.isci.2021.102690 NCBI Sequence Read Archive:PRJNA887020 Software and Algorithms Prism GraphPad Version#8 Immunarch R package Version#0.6.7 Metascape www.metascape.org N/A Attune NxT Flow Cytometer Software ThermoFisher Scientific Version#2.5 FlowJo TreeStar Version#10.5 Ideas Analysis Software Luminex Corporation Version#6.1 Seahorse Wave Agilent Technologies Version#2.6.1 xPONENT Software for Luminex Instruments Luminex Corporation Version#4.3 Cell Ranger Single-Cell Software Suite 10X Genomics Version 6.0.2 Seurat R package Version 3.1.5 Monocle R package Version 2.8.0

Techniques: Isolation, Gene Expression